CCA tRNA nucleotidyltransferase

CCA tRNA nucleotidyltransferase (EC 2.7.7.72, CCA-adding enzyme, tRNA adenylyltransferase, tRNA CCA-pyrophosphorylase, tRNA-nucleotidyltransferase, transfer-RNA nucleotidyltransferase, transfer ribonucleic acid nucleotidyl transferase, CTP(ATP):tRNA nucleotidyltransferase, transfer ribonucleate adenylyltransferase, transfer ribonucleate adenyltransferase, transfer RNA adenylyltransferase, transfer ribonucleate nucleotidyltransferase, ATP (CTP):tRNA nucleotidyltransferase, ribonucleic cytidylic cytidylic adenylic pyrophosphorylase, transfer ribonucleic adenylyl (cytidylyl) transferase, transfer ribonucleic-terminal trinucleotide nucleotidyltransferase, transfer ribonucleate cytidylyltransferase, ribonucleic cytidylyltransferase, -C-C-A pyrophosphorylase, ATP(CTP)-tRNA nucleotidyltransferase, tRNA adenylyl(cytidylyl)transferase, CTP:tRNA cytidylyltransferase) is an enzyme with systematic name CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

a tRNA precursor + 2 CTP + ATP

\rightleftharpoons

a tRNA with a 3' CCA end + 3 diphosphate (overall reaction)

(1a) a tRNA precursor + CTP

\rightleftharpoons

a tRNA with a 3' cytidine end + diphosphate

(1b) a tRNA with a 3' cytidine + CTP

\rightleftharpoons

a tRNA with a 3' CC end + diphosphate

(1c) a tRNA with a 3' CC end + ATP

\rightleftharpoons

a tRNA with a 3' CCA end + diphosphate

The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence.

References

↑ Schofield, P.; Williams, K.R. (1977). "Purification and some properties of Escherichia coli tRNA nucleotidyltransferase". J. Biol. Chem. 252 (15): 5584–5588. PMID 328503.
↑ Shi, P.Y.; Maizels, N.; Weiner, A.M. (1998). "CCA addition by tRNA nucleotidyltransferase: polymerization without translocation". EMBO J. 17 (11): 3197–3206. doi:10.1093/emboj/17.11.3197. PMID 9606201.
↑ Augustin, M.A.; Reichert, A.S.; Betat, H.; Huber, R.; Morl, M.; Steegborn, C. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328: 985–994. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736.
↑ Yakunin, A.F.; Proudfoot, M.; Kuznetsova, E.; Savchenko, A.; Brown, G.; Arrowsmith, C.H.; Edwards, A.M. (2004). "The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2′,3′-cyclic phosphodiesterase, 2′-nucleotidase, and phosphatase activities". J. Biol. Chem. 279 (35): 36819–36827. doi:10.1074/jbc.M405120200. PMID 15210699.
↑ Hou, Y.M. (2010). "CCA addition to tRNA: implications for tRNA quality control". IUBMB Life. 62 (4): 251–260. doi:10.1002/iub.301. PMID 20101632.

External links

CCA tRNA nucleotidyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferases: phosphorus-containing groups (EC 2.7)

2.7.1-2.7.4:
phosphotransferase/kinase
(PO₄)

2.7.1: OH acceptor	Hexo- Gluco- Fructo- Hepatic Galacto- Phosphofructo- 1 Liver Muscle Platelet 2 Riboflavin Shikimate Thymidine ADP-thymidine NAD⁺ Glycerol Pantothenate Mevalonate Pyruvate Deoxycytidine PFP Diacylglycerol Phosphoinositide 3 Class I PI 3 Class II PI 3 Sphingosine Glucose-1,6-bisphosphate synthase

2.7.2: COOH acceptor	Phosphoglycerate Aspartate kinase

2.7.3: N acceptor	Creatine

2.7.4: PO₄ acceptor	Phosphomevalonate Adenylate Nucleoside-diphosphate Uridylate Guanylate Thiamine-diphosphate

2.7.6: diphosphotransferase
(P₂O₇)

2.7.7: nucleotidyltransferase
(PO₄-nucleoside)

Polymerase

DNA polymerase	DNA-directed DNA polymerase I II III IV V RNA-directed DNA polymerase Reverse transcriptase Telomerase DNA nucleotidylexotransferase/Terminal deoxynucleotidyl transferase

RNA nucleotidyltransferase	RNA polymerase/DNA-directed RNA polymerase RNA polymerase I RNA polymerase II RNA polymerase III RNA polymerase IV Primase RNA-dependent RNA polymerase PNPase

Phosphorolytic
3' to 5' exoribonuclease

Nucleotidyltransferase

Guanylyltransferase

mRNA capping enzyme

Other

2.7.8: miscellaneous

Phosphatidyltransferases	CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase CDP-diacylglycerol—serine O-phosphatidyltransferase CDP-diacylglycerol—inositol 3-phosphatidyltransferase CDP-diacylglycerol—choline O-phosphatidyltransferase

Glycosyl-1-phosphotransferase	N-acetylglucosamine-1-phosphate transferase

2.7.10-2.7.13: protein kinase
(PO₄; protein acceptor)

2.7.10: protein-tyrosine	see tyrosine kinases

2.7.11: protein-serine/threonine	see serine/threonine-specific protein kinases

2.7.12: protein-dual-specificity	see serine/threonine-specific protein kinases

2.7.13: protein-histidine	Protein-histidine pros-kinase Protein-histidine tele-kinase Histidine kinase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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CCA tRNA nucleotidyltransferase

References

See also

External links