Rhamnogalacturonan endolyase

Rhamnogalacturonan endolyase
Identifiers
EC number 4.2.2.23
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Rhamnogalacturonan endolyase (EC 4.2.2.23, rhamnogalacturonase B, alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase, Rgase B, rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase, RG-lyase, YesW, RGL4, Rgl11A, Rgl11Y, RhiE) is an enzyme with systematic name alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronate endolyase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction

Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.

The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168 and Aspergillus aculeatus.

References

  1. ↑ Mutter, M.; Colquhoun, I.J.; Schols, H.A.; Beldman, G.; Voragen, A.G. (1996). "Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase". Plant Physiol. 110: 73–77. doi:10.1104/pp.110.1.73. PMID 8587995.
  2. ↑ Azadi, P.; O'Neill, M.A.; Bergmann, C.; Darvill, A.G.; Albersheim, P. (1995). "The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase". Glycobiology. 5: 783–789. doi:10.1093/glycob/5.8.783. PMID 8720076.
  3. ↑ Mutter, M.; Colquhoun, I.J.; Beldman, G.; Schols, H.A.; Bakx, E.J.; Voragen, A.G. (1998). "Characterization of recombinant rhamnogalacturonan α-L-rhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin". Plant Physiol. 117: 141–152. doi:10.1104/pp.117.1.141. PMID 9576783.
  4. ↑ Kadirvelraj, R.; Harris, P.; Poulsen, J.C.; Kauppinen, S.; Larsen, S. (2002). "A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus". Acta Crystallogr. D. 58: 1346–1349. doi:10.1107/s0907444902009137. PMID 12136151.
  5. ↑ Laatu, M.; Condemine, G. (2003). "Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi". J. Bacteriol. 185: 1642–1649. doi:10.1128/jb.185.5.1642-1649.2003. PMID 12591882.
  6. ↑ Pages, S.; Valette, O.; Abdou, L.; Belaich, A.; Belaich, J.P. (2003). "A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome". J. Bacteriol. 185: 4727–4733. doi:10.1128/jb.185.16.4727-4733.2003. PMID 12896991.
  7. ↑ Ochiai, A.; Yamasaki, M.; Itoh, T.; Mikami, B.; Hashimoto, W.; Murata, K. (2006). "Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 438–440. doi:10.1107/s1744309106011894. PMID 16682770.
  8. ↑ Jensen, M.H.; Otten, H.; Christensen, U.; Borchert, T.V.; Christensen, L.L.; Larsen, S.; Leggio, L.L. (2010). "Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus". J. Mol. Biol. 404: 100–111. doi:10.1016/j.jmb.2010.09.013. PMID 20851126.
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