Exodeoxyribonuclease (lambda-induced)
| Exodeoxyribonuclease (lambda-induced) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.1.11.3 | ||||||||
| CAS number | 37367-70-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Exodeoxyribonuclease (lambda-induced) (EC 3.1.11.3, lambda exonuclease, phage lambda-induced exonuclease, Escherichia coli exonuclease IV, E. coli exonuclease IV, exodeoxyribonuclease IV, exonuclease IV) is an exonuclease.[1][2] This enzyme catalyses the following chemical reaction
- Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
This enzyme has preference for double-stranded DNA (dsDNA). This means that it degrades a single strand of dsDNA, primarily any strand which has a phosphate at its 5' end. [3]
References
- ↑ Lindahl, T.; Gally, J.A.; Edelman, G.M. (1969). "Deoxyribonuclease IV: a new exonuclease from mammalian tissues". Proc. Natl. Acad. Sci. USA. 62: 597–603. doi:10.1073/pnas.62.2.597. PMC 277851
. PMID 5256235. - ↑ Little, J.W. (1967). "An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction". J. Biol. Chem. 242 (4): 679–686. PMID 6017737.
- ↑ http://www.epibio.com/docs/default-source/protocols/lambda-exonuclease.pdf
External links
- Exodeoxyribonuclease (lambda-induced) at the US National Library of Medicine Medical Subject Headings (MeSH)
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