TRNA-dihydrouridine20 synthase (NAD(P)+)

TRNA-dihydrouridine20 synthase (NAD(P)⁺) (EC 1.3.1.91, Dus2p, tRNA-dihydrouridine synthase 2) is an enzyme with systematic name tRNA-5,6-dihydrouracil20:NAD(P)⁺ oxidoreductase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

5,6-dihydrouracil20 in tRNA + NAD(P)⁺

\rightleftharpoons

uracil20 in tRNA + NAD(P)H + H⁺

This enzyme specifically modifies uracil20 in tRNA.

References

↑ Xing, F.; Hiley, S.L.; Hughes, T.R.; Phizicky, E.M. (2004). "The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs". J. Biol. Chem. 279: 17850–17860. doi:10.1074/jbc.M401221200. PMID 14970222.
↑ Xing, F.; Martzen, M.R.; Phizicky, E.M. (2002). "A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA". RNA. 8: 370–381. doi:10.1017/S1355838202029825. PMC 1370258. PMID 12003496.
↑ Rider, L.W.; Ottosen, M.B.; Gattis, S.G.; Palfey, B.A. (2009). "Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction". J. Biol. Chem. 284: 10324–10333. doi:10.1074/jbc.M806137200. PMC 2667719. PMID 19139092.
↑ Kato, T.; Daigo, Y.; Hayama, S.; Ishikawa, N.; Yamabuki, T.; Ito, T.; Miyamoto, M.; Kondo, S.; Nakamura, Y. (2005). "A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis". Cancer Res. 65: 5638–5646. doi:10.1158/0008-5472.CAN-05-0600. PMID 15994936.

Oxidoreductases: CH–CH oxidoreductases (EC 1.3)

1.3.1: NAD/NADP acceptor	Enoyl-acyl carrier protein reductase/Enoyl ACP reductase 7-Dehydrocholesterol reductase Biliverdin reductase 2,4 Dienoyl-CoA reductase Dihydroxymethyloxo-tetrahydroquinoline dehydrogenase

1.3.3: Oxygen acceptor	Dihydroorotate dehydrogenase Coproporphyrinogen III oxidase Protoporphyrinogen oxidase Bilirubin oxidase Acyl-CoA oxidase Dihydrouracil oxidase Tetrahydroberberine oxidase Secologanin synthase Tryptophan alpha,beta-oxidase Pyrroloquinoline-quinone synthase L-galactonolactone oxidase

1.3.5: Quinone	Succinate dehydrogenase SDHA SDHB SDHC SDHD

1.3.99: Other acceptors	Fumarate reductase Butyryl-CoA dehydrogenase Acyl CoA dehydrogenase ACADSB ACADS 5α-reductase SRD5A1 SRD5A2 SRD5A3 Glutaryl-CoA dehydrogenase Isovaleryl coenzyme A dehydrogenase 3-oxo-5beta-steroid 4-dehydrogenase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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