para-Nitrophenylphosphate

para-Nitrophenylphosphate

Names

(4-nitrophenyl) dihydrogen phosphate

Other names

pNPP

Identifiers

3D model (Jmol)

ECHA InfoCard

InChI=1S/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)^N
Key: XZKIHKMTEMTJQX-UHFFFAOYSA-N^N
InChI=1/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)
Key: XZKIHKMTEMTJQX-UHFFFAOYAU

Properties

C₆H₆NO₆P

219.088701

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

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Infobox references

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.

{\ce {{PNPP}+H2O->[_{AP}]{PNP}+P_{i}}}

External links

This article is issued from Wikipedia - version of the 6/7/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.