French flag model
The French flag model is a conceptual definition of a morphogen, described by Lewis Wolpert in the 1960s. A morphogen is defined as a signaling molecule that acts directly on cells (not through serial induction) to produce specific cellular responses dependent on morphogen concentration. During early development, morphogen gradients generate different cell types in distinct spatial order.
In the French flag model, the French flag is used to represent the effect of a morphogen on cell differentiation: a morphogen affects cell states based on concentration, these states are represented by the different colors of the French flag: high concentrations activate a "blue" gene, lower concentrations activate a "white" gene, with "red" serving as the default state in cells below the necessary concentration threshold.
The French Flag model was championed by the leading Drosophila biologist, Peter Lawrence. Christiane Nüsslein-Volhard identified the first morphogen, Bicoid, one of the transcription factors present in a gradient in the Drosophila syncytial embryo. Two labs, that of Gary Struhl and that of Stephen Cohen, then demonstrated that a secreted signaling protein, Decapentaplegic (the Drosophila homologue of Transforming Growth Factor Beta), acted as a morphogen during later stages of Drosophila development. substance governing the pattern of tissue development and, in particular, the positions of the various specialized cell types within a tissue. It spreads from a localized source and forms a concentration gradient across a developing tissue.
Some of the earliest and best-studied morphogens are transcription factors that diffuse within early Drosophila melanogaster (fruit fly) embryos. However, most morphogens are secreted proteins that signal between cells.
Morphogens are defined conceptually, not chemically, so simple chemicals such as retinoic acid may also act as morphogens.
Difficulties with the French Flag Model
The basis of the French Flag model is the idea that a morphogen autonomously forms a gradient with individual cells reading the concentration of the gradient. Cells then respond to a specific level of gradient with a specific differentiation to match the position the gradient indicates they are in. While widely accepted as an important model for understanding morphogenesis, it is not universally accepted by all developmental biologists. The difficulties with all gradient based models of morphogenesis were extensively reviewed by Richard Gordon (theoretical biologist) and include seven specific points:
1) In order to maintain a gradient at steady state there has to be a sink i.e. a way in which diffusing molecules are destroyed or removed along the way and/or at some boundaries. Sinks are rarely, if ever, even considered when the gradient model is invoked.
2) Diffusion must occur in a confined space, if a gradient is to be established however many organisms such as the axolotl develop normally even if the vitelline membrane and jelly layers are removed and development occurs in flowing water.
3) Diffusion is temperature dependent yet development can proceed normally over a wide variety of temperatures in animals whose eggs develop external to the mother.
4) Diffusion gradients do not scale well yet embryos come in variety of sizes.
5) Diffusion gradients follow the superposition principle. This means that a gradient of one substance in one direction, and a gradient of the same substance in a perpendicular direction, result in a single one-dimensional gradient in the diagonal direction, not a two dimensional gradient. Developmental biologists frequently invoke a two dimensional gradient even though a two dimensional gradient system requires two morphogen gradients with two different sources and sinks placed approximately perpendicular to one another.
6) Fluctuations in gradients always occur, especially at the low concentrations commonly found during embryogenesis, making a specific response by an individual cell to particular concentration thresholds problematic.
7) Each cell has to be able to “read” the morphogen concentration accurately, otherwise boundaries between tissues become ragged. Yet such ragged boundaries are rare in development.
- Knabe J.F; et al. (2008). Evolution and Morphogenesis of Differentiated Multicellular Organisms: Autonomously Generated Diffusion Gradients for Positional Information. Artificial Life XI: Proceedings of the Eleventh International Conference on the Simulation and Synthesis of Living Systems.
- Wolpert L (October 1969). "Positional information and the spatial pattern of cellular differentiation". J. Theor. Biol. 25 (1): 1–47. doi:10.1016/S0022-5193(69)80016-0. PMID 4390734.
- Wolpert, Lewis; et al. (2007). Principles of development (3rd ed.). Oxford [Oxfordshire]: Oxford University Press. ISBN 0-19-927536-X.
- Gordon, NK,Gordon,R. The organelle of differentiation in embryos: the cell state splitter Theoretical Biology and Medical Modelling, 13(11) 2016
Excellent resources for further study of this topic include:
- Interactive Fly
- Artificial Life model of multicellular morphogenesis with autonomously generated gradients for positional information