Francine John-Calame (born April 30, 1954) is a Swiss politician from the Canton of Neuchâtel and a member of the National Council. She was member of the parliament of the Canton of Neuchâtel from 1993 to 2002. She is a municipal councillor in Le Cerneux-Péquignot.
On the list of the Green Party, she acceeded to the National Council on May 31, 2005 following the resignation of Fernand Cuche.
Francine John-Calame is married and mother of two.