Flavonoid 3',5'-hydroxylase
| Flavanoid 3',5'-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.88 | ||||||||
| CAS number | 94047-23-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Flavanoid 3',5'-hydroxylase (EC 1.14.13.88) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase.[1][2][3] This enzyme catalyses the following chemical reaction
- flavanone + 2 NADPH + 2 H+ + 2 O2 3',5'-dihydroxyflavanone + 2 NADP+ + 2 H2O (overall reaction)
- (1a) flavanone + NADPH + H+ + O2 3'-hydroxyflavanone + NADP+ + H2O
- (1b) 3'-hydroxyflavanone + NADPH + H+ + O2 3',5'-dihydroxyflavanone + NADP+ + H2O
Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).
References
- ↑ Menting, J.; Scopes, R.K.; Stevenson, T.W. (1994). "Characterization of flavonoid 3′,5′-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers". Plant Physiol. 106 (2): 633–642. doi:10.1104/pp.106.2.633. PMC 159570
. PMID 12232356. - ↑ Shimada, Y.; Nakano-Shimada, R.; Ohbayashi, M.; Okinaka, Y.; Kiyokawa, S.; Kikuchi, Y. (1999). "Expression of chimeric P450 genes encoding flavonoid-3′, 5′-hydroxylase in transgenic tobacco and petunia plants1". FEBS Lett. 461 (3): 241–245. doi:10.1016/s0014-5793(99)01425-8. PMID 10567704.
- ↑ de Vetten, N.; ter Horst, J.; van Schaik, H.P.; de Boer, A.; Mol, J.; Koes, R. (1999). "A cytochrome b5 is required for full activity of flavonoid 3′, 5′-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors". Proc. Natl. Acad. Sci. USA. 96 (2): 778–783. doi:10.1073/pnas.96.2.778. PMC 15213. PMID 9892710.
External links
- Flavanoid 3',5'-hydroxylase at the US National Library of Medicine Medical Subject Headings (MeSH)
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