Cyanophycin synthase (L-arginine-adding)

Cyanophycin synthase (L-arginine-adding)
Identifiers
EC number 6.3.2.30
CAS number 131554-17-1
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Cyanophycin synthase (L-arginine-adding) (EC 6.3.2.30, CphA, CphA1, CphA2, cyanophycin synthetase, multi-L-arginyl-poly-L-aspartate synthase) is an enzyme with systematic name cyanophycin:L-arginine ligase (ADP-forming).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg ADP + phosphate + [L-Asp(4-L-Arg)]n+1

This enzyme requires Mg2+ for activity.

References

  1. Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A. (2000). "Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308". Arch. Microbiol. 174 (5): 297–306. doi:10.1007/s002030000206. PMID 11131019.
  2. Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A. (2001). "Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity". Appl. Environ. Microbiol. 67: 2176–2182. doi:10.1128/AEM.67.5.2176-2182.2001. PMID 11319097.
  3. Allen, M.M.; Hutchison, F.; Weathers, P.J. (1980). "Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308". J. Bacteriol. 141 (2): 687–693. PMID 6767688.
  4. Berg, H.; Ziegler, K.; Piotukh, K.; Baier, K.; Lockau, W.; Volkmer-Engert, R. (2000). "Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers". Eur. J. Biochem. 267 (17): 5561–5570. doi:10.1046/j.1432-1327.2000.01622.x. PMID 10951215.
  5. Ziegler, K.; Deutzmann, R.; Lockau, W. (2002). "Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense". Z. Naturforsch. C. 57 (5-6): 522–529. doi:10.1515/znc-2002-5-621. PMID 12132696.
  6. Ziegler, K.; Diener, A.; Herpin, C.; Richter, R.; Deutzmann, R.; Lockau, W. (1998). "Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin)". Eur. J. Biochem. 254: 154–159. doi:10.1046/j.1432-1327.1998.2540154.x. PMID 9652408.

External links

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