Available structures
PDBOrtholog search: PDBe RCSB
Aliases CALCRL, CGRPR, CRLR, calcitonin receptor like receptor
External IDs MGI: 1926944 HomoloGene: 21179 GeneCards: CALCRL
RNA expression pattern

More reference expression data
Species Human Mouse









RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 2: 187.34 – 187.45 Mb Chr 2: 84.33 – 84.43 Mb
PubMed search [1] [2]
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Calcitonin receptor-like (CALCRL), also known as the calcitonin receptor-like receptor (CRLR), is a human protein.[3]


The protein encoded by the CALCRL gene is a G protein-coupled receptor related to the calcitonin receptor. CALCRL is linked to one of three single transmembrane domain receptor activity-modifying proteins (RAMPs) that are essential for functional activity.

The association of CALCRL with different RAMP proteins produces different receptors:[4][5]

These receptors are linked to the G protein Gs,[7] which activates adenylate cyclase and activation results in the generation of intracellular cyclic adenosine monophosphate (cAMP).


CALCRL associated with RAMP 1 produces the CGRP receptor which is a trans-membrane protein receptor that is made up of four chains. Two of the four chains contain unique sequences. It is a heterodimer protein composed of two polypeptide chains differing in composition of their amino acid residues. The sequence reveals multiple hydrophobic and hydrophilic regions throughout the four chains in the protein.[8]

The structural dynamics of an active state complex of the Calcitonin receptor-like protein, CGRP, the Receptor Activity-Modifying Protein 1, and the G-protein C-terminus has been elucidated using a computational and experimental approach.[9]

Clinical significance

These G-protein coupled receptor (GPCRs) proteins play an important role in pharmaceutical targets. Many drugs used today alter the GPCR signaling pathways,[8] notably calcitonin gene-related peptide receptor antagonists that are under investigation for the treatment of migraine.


  1. "Human PubMed Reference:".
  2. "Mouse PubMed Reference:".
  3. Aiyar N, Rand K, Elshourbagy NA, Zeng Z, Adamou JE, Bergsma DJ, Li Y (May 1996). "A cDNA encoding the calcitonin gene-related peptide type 1 receptor". J. Biol. Chem. 271 (19): 11325–9. doi:10.1074/jbc.271.19.11325. PMID 8626685.
  4. McLatchie LM, Fraser NJ, Main MJ, Wise A, Brown J, Thompson N, Solari R, Lee MG, Foord SM (May 1998). "RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor". Nature. 393 (6683): 333–9. doi:10.1038/30666. PMID 9620797.
  5. Foord SM, Marshall FH (May 1999). "RAMPs: accessory proteins for seven transmembrane domain receptors". Trends Pharmacol. Sci. 20 (5): 184–7. doi:10.1016/S0165-6147(99)01347-4. PMID 10354609.
  6. Kamitani S, Asakawa M, Shimekake Y, Kuwasako K, Nakahara K, Sakata T (April 1999). "The RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells". FEBS Lett. 448 (1): 111–4. doi:10.1016/S0014-5793(99)00358-0. PMID 10217420.
  7. "Receptor properties". SenseLab Project: Membrane properties resource. Yale University. Retrieved 2008-09-28.
  8. 1 2 PDB: 3N7S; ter Haar E, Koth CM, Abdul-Manan N, Swenson L, Coll JT, Lippke JA, Lepre CA, Garcia-Guzman M, Moore JM (2010). "Crystal structure of the ectodomain complex of the CGRP receptor, a class-B GPCR, reveals the site of drug antagonism". Structure. 18 (9): 1083–93. doi:10.1016/j.str.2010.05.014. PMID 20826335.
  9. Weston C, Winfield I, Harris M, Hodgson R, Shah A, Dowell SJ, Mobarec JC, Woodcock DA, Reynolds CA, Poyner DR, Watkins HA, Ladds G (August 2016). "Receptor activity modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors receptor". The Journal of Biological Chemistry. doi:10.1074/jbc.M116.751362. PMID 27566546.

Further reading

  • Born W, Muff R, Fischer JA (2002). "Functional interaction of G protein-coupled receptors of the adrenomedullin peptide family with accessory receptor-activity-modifying proteins (RAMP).". Microsc. Res. Tech. 57 (1): 14–22. doi:10.1002/jemt.10051. PMID 11921352. 
  • Yallampalli C, Chauhan M, Thota CS, et al. (2003). "Calcitonin gene-related peptide in pregnancy and its emerging receptor heterogeneity.". Trends Endocrinol. Metab. 13 (6): 263–9. doi:10.1016/s1043-2760(02)00563-5. PMID 12128288. 
  • Foord SM, Craig RK (1988). "Isolation and characterisation of a human calcitonin-gene-related-peptide receptor.". Eur. J. Biochem. 170 (1-2): 373–9. doi:10.1111/j.1432-1033.1987.tb13710.x. PMID 2826160. 
  • Skofitsch G, Jacobowitz DM (1986). "Autoradiographic distribution of 125I calcitonin gene-related peptide binding sites in the rat central nervous system.". Peptides. 6 (5): 975–86. doi:10.1016/0196-9781(85)90331-6. PMID 3001670. 
  • Flühmann B, Muff R, Hunziker W, et al. (1995). "A human orphan calcitonin receptor-like structure.". Biochem. Biophys. Res. Commun. 206 (1): 341–7. doi:10.1006/bbrc.1995.1047. PMID 7818539. 
  • Aiyar N, Rand K, Elshourbagy NA, et al. (1996). "A cDNA encoding the calcitonin gene-related peptide type 1 receptor.". J. Biol. Chem. 271 (19): 11325–9. doi:10.1074/jbc.271.19.11325. PMID 8626685. 
  • McLatchie LM, Fraser NJ, Main MJ, et al. (1998). "RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor.". Nature. 393 (6683): 333–9. doi:10.1038/30666. PMID 9620797. 
  • Sams A, Jansen-Olesen I (1999). "Expression of calcitonin receptor-like receptor and receptor-activity-modifying proteins in human cranial arteries.". Neurosci. Lett. 258 (1): 41–4. doi:10.1016/S0304-3940(98)00844-1. PMID 9876047. 
  • Kamitani S, Asakawa M, Shimekake Y, et al. (1999). "The RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells.". FEBS Lett. 448 (1): 111–4. doi:10.1016/S0014-5793(99)00358-0. PMID 10217420. 
  • Aldecoa A, Gujer R, Fischer JA, Born W (2000). "Mammalian calcitonin receptor-like receptor/receptor activity modifying protein complexes define calcitonin gene-related peptide and adrenomedullin receptors in Drosophila Schneider 2 cells.". FEBS Lett. 471 (2-3): 156–60. doi:10.1016/S0014-5793(00)01387-9. PMID 10767413. 
  • Frayon S, Cueille C, Gnidéhou S, et al. (2000). "Dexamethasone increases RAMP1 and CRLR mRNA expressions in human vascular smooth muscle cells.". Biochem. Biophys. Res. Commun. 270 (3): 1063–7. doi:10.1006/bbrc.2000.2552. PMID 10772950. 
  • Kuwasako K, Shimekake Y, Masuda M, et al. (2000). "Visualization of the calcitonin receptor-like receptor and its receptor activity-modifying proteins during internalization and recycling.". J. Biol. Chem. 275 (38): 29602–9. doi:10.1074/jbc.M004534200. PMID 10882736. 
  • Evans BN, Rosenblatt MI, Mnayer LO, et al. (2000). "CGRP-RCP, a novel protein required for signal transduction at calcitonin gene-related peptide and adrenomedullin receptors.". J. Biol. Chem. 275 (40): 31438–43. doi:10.1074/jbc.M005604200. PMID 10903324. 
  • Hilairet S, Foord SM, Marshall FH, Bouvier M (2001). "Protein-protein interaction and not glycosylation determines the binding selectivity of heterodimers between the calcitonin receptor-like receptor and the receptor activity-modifying proteins.". J. Biol. Chem. 276 (31): 29575–81. doi:10.1074/jbc.M102722200. PMID 11387328. 
  • Kamitani S, Sakata T (2001). "Glycosylation of human CRLR at Asn123 is required for ligand binding and signaling.". Biochim. Biophys. Acta. 1539 (1-2): 131–9. doi:10.1016/S0167-4889(01)00100-8. PMID 11389975. 
  • Nikitenko LL, Brown NS, Smith DM, et al. (2001). "Differential and cell-specific expression of calcitonin receptor-like receptor and receptor activity modifying proteins in the human uterus.". Mol. Hum. Reprod. 7 (7): 655–64. doi:10.1093/molehr/7.7.655. PMID 11420389. 
  • Hilairet S, Bélanger C, Bertrand J, et al. (2001). "Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin.". J. Biol. Chem. 276 (45): 42182–90. doi:10.1074/jbc.M107323200. PMID 11535606. 
  • Aiyar N, Disa J, Pullen M, Nambi P (2002). "Receptor activity modifying proteins interaction with human and porcine calcitonin receptor-like receptor (CRLR) in HEK-293 cells.". Mol. Cell. Biochem. 224 (1-2): 123–33. doi:10.1023/A:1011907328682. PMID 11693189. 
  • Hagner S, Haberberger RV, Overkamp D, et al. (2002). "Expression and distribution of calcitonin receptor-like receptor in human hairy skin.". Peptides. 23 (1): 109–16. doi:10.1016/S0196-9781(01)00586-1. PMID 11814625. 
  • Hill H, Pioszak A (2013). "Bacterial expression and purification of a heterodimeric adrenomedullin receptor extracellular domain complex using DsbC-assisted disulfide shuffling.". Protein Expr Purif. 88 (1): 107–13. doi:10.1016/j.pep.2012.11.019. PMID 23247088. 

External links

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