(R)-6-hydroxynicotine oxidase

The 3 substrates of this enzyme are (R)-6-hydroxynicotine, H₂O, and O₂, whereas its two products are 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is (R)-6-hydroxynicotine:oxygen oxidoreductase. Other names in common use include D-6-hydroxynicotine oxidase, and 6-hydroxy-D-nicotine oxidase. It employs one cofactor, FAD.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2BVF, 2BVG, and 2BVH.

References

Bruhmuller M, Mohler H, Decker K (1972). "Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase". Eur. J. Biochem. 29 (1): 143–51. doi:10.1111/j.1432-1033.1972.tb01968.x. PMID 4628374.
Decker K, Bleeg H (1965). "Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans". Biochim. Biophys. Acta. 105 (2): 313–24. doi:10.1016/s0926-6593(65)80155-2. PMID 5849820.

Oxidoreductases: CH-NH (EC 1.5)

1.5.1: NAD or NADP acceptor	Dihydrofolate reductase Saccharopine dehydrogenase Methylenetetrahydrofolate reductase

1.5.3: oxygen acceptor	Dihydrobenzophenanthridine oxidase Sarcosine oxidase Proline oxidase

1.5.5: quinone acceptor	Electron-transferring-flavoprotein dehydrogenase

1.5.99	Sarcosine dehydrogenase Cytokinin dehydrogenase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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