Acireductone synthase

Acireductone synthase (EC 3.1.3.77, E1, E-1 enolase-phosphatase) is an enzyme with systematic name 5-(methylthio)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

5-(methylthio)-2,3-dioxopentyl phosphate + H₂O

\rightleftharpoons

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate (overall reaction)

(1a) 5-(methylthio)-2,3-dioxopentyl phosphate

\rightleftharpoons

2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (probably spontaneous)

(1b) 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H₂O

\rightleftharpoons

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate

References

↑ Myers, R.W.; Wray, J.W.; Fish, S.; Abeles, R.H. (1993). "Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae". J. Biol. Chem. 268 (33): 24785–24791. PMID 8227039.
↑ Wray, J.W.; Abeles, R.H. (1995). "The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases". J. Biol. Chem. 270: 3147–3153. doi:10.1074/jbc.270.7.3147. PMID 7852397.
↑ Wang, H.; Pang, H.; Bartlam, M.; Rao, Z. (2005). "Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity". J. Mol. Biol. 348 (4): 917–926. doi:10.1016/j.jmb.2005.01.072. PMID 15843022.

Acireductone synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

3.1.1: Carboxylic
ester hydrolases

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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